Multiplex PCR screening for virulence genes of Klebsiella pneumoniae isolated from microbiota of diseased and healthy people

Introduction . Klebsiella pneumoniae in human microbiota may appear as a part of commensal microbiota, and hypervirulent pathogen, for example, hypermucoid pathotype. This pathotype is characterized by certain genetic determinants, leading to the possibility detecting pathogenic potential isolates PCR. Aim study : evaluate compare K. from practically healthy people, patients with inflammatory bowel disease (IBD) extraintestinal infections (ExII). Materials methods Testing was performed set nucleotides multiplex PCR analysis targeting eight potentially virulent genes following functions: ferrum uptake (ybsT, kfu, iutA), adhesion invasion (mrkD), phenotype serotypes (mrkD, magA, rmpA, k2) metabolism allantoin (allS). assay used screen feces IBD (69 isolates) people (68 isolates), multiresistant biological material (blood, urine, surgical wounds, bronchoalveolar lavage) (mrExII, 25 isolates). Results. Results testing demonstrated association four targeted determinants diagnoses. YbtS gene significantly more often found (р = 0.024) mrExII (p < 0.001) groups. RmpA detected group 0.038). Extraintestinal infectious were ≤ enriched mrkD allS 0.032). Conclusion The most isolated infections, least — people. frequently virulence involved formation.